Exp 1: Determination of free fatty acid content in fats and oils

Make yourself familiar with the instruments & glassware (hover for more info)

Measuring cylinder Conical Flask Weighing Scale Hot plate Burette

Step 1: Select the sample

Select This Sample
Select This Sample
Select This Sample
Select This Sample

Which of the sample has darkest color?

Step 2: Weigh 2gms of oil sample in conical flask

click to hold sample
click to place flask
0.0

What is the required quantity of oil to be taken for % FFA content?

Step 3: Add 50 ml ethyl alcohol in conical flask

click to pour alcohol
click to pour alcohol

What is the required quantity of oil to be taken for % FFA content?

Step 4: Heat the mixture upto boiling condition

⚠ Please proceed with caution while working with burner

click to turn on hot plate
click to place flask

Step 5: Add 2-3 drops of phenolpthalein indicator in hot condition

click to pour phenolphthalein

Step 6: Titrate the mixture with 0.1 N NaOH solution

0 ml

click to pour 0.1N NaOH

Which type of titration has been used to determine % FFA

Observation Table


Selected sample Weight of sample (g) (W) Normality of NaOH (N) Burette reading (ml) Titrant used (ml)
Initial value Final value
2.0 0.1 0.0


FFA(%) =

FFA (%, Oleic acid) = V x N x 28.2
W
FFA (%, Oleic acid) = x 0.1 x 28.2
2
FFA (%, Oleic acid) =

Inference

The maximum permissible limit of FFA as per FSSAI in the edible oil is 0.5%

The limit of FFA as per FSSAI standard is 0.05%. The FFA content of the given sample is 0.28%.
So, this sample is in the FSSAI range and safe for edible purposes.