ESTIMATION of copper(ii) by spectrocolorimetry

Aim:

To verify the Beer-Lamberts law and estimation of copper (II) in the given solution by spectrocolorimetry.


Apparatus used:

Calorimeter, Conical flask, Test tube, Beaker, Dropper etc.


 Solutions used Ethyl Diamine Tetra-Acetic Acid (EDTA) solution, Water sample, Calcium carbonate solution, Ammonia buffer solution, Eriochrome Black T (EBT) as indicator Description According to Beer-Lamberts law the optical density of absorbance of a solution of concentration ‘c’ mol dm-3 placed in a cell of ‘b’ cm width is given by 
          A = εbc
where ε is called the molar absorption coefficient or molar extinction coefficient. The absorbance A is defined as
A = log (I0/I) .
where I0 and I represents the intensities of incident and transmitted radiations since the optical density is linearly proportional to the concentration of the solution, a linear plot is expected for absorbance Vs concentration. . Instructions 1.. 2.. CuSO 4 Water(200ml) NH 4 NO 3 (10%)